Open AccessIsolation And Identification of Secondary Methabolites Compound of Moringa Oleifera Lamk Leaf Acetic Ethyl Extract
Author(s) -
Sudding,
Netti Herawati
Publication year - 2021
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1752/1/012053
Subject(s) - moringa , chloroform , ethyl acetate , chemistry , solvent , solubility , fourier transform infrared spectroscopy , fractionation , chromatography , extraction (chemistry) , nuclear chemistry , organic chemistry , food science , physics , quantum mechanics
This study aims to isolate and identify secondary metabolite compounds in the ethyl acetate extract of Moringa leaves ( Moringa oleifera Lamk). This isolation includes several stages, namely extraction, fractionation, purification and identification. Identification includes solubility test, UV-VIS spectroscopy test, FTIR and GCMS. Based on the solubility test results, it is known that the isolate is soluble in ethyl acetate solvent and dissolves well in the chloroform solvent, this shows that the isolate obtained is semi-polar and the compound glows under UV light. UV-VIS spectroscopy test results showed a maximum wavelength of 210.52 nm showing the characteristics of the ester group. FTIR spectroscopy data showed wave numbers (cm-1) namely: 3450.65 (OH alcohol), 2926.01 and 2560.43 (C-H); 1456.26 and 1382.96 (CH2 and CH3), 1722.43 (C=O); 1616.26 (C = C aromatic); 1039,63 (C-O). The result of GC-MS showed molecular weight of compound is 166. From the results of the analysis it was suggested that the isolate was a phenolic group that are 4-hydroxy benzoyl ethyl esthers.