Open AccessThe effect of coconut milk in various concentrations on spermatozoa motility of Koi fish (Cyprinus carpio, Linnaeus 1758) 48 hours post-cryopreservation
Author(s) -
Amanda Devita,
. Abinawanto,
Retno Lestari
Publication year - 2021
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1725/1/012061
Subject(s) - milt , cyprinus , cryopreservation , biology , zoology , cryoprotectant , significant difference , fish <actinopterygii> , fishery , mathematics , embryo , statistics
Research about the effect of various concentrations of coconut milk on spermatozoa quality of Koi Fish ( Cyprinus carpio , Linnaeus 1758) 48 hours post cryopreservation has been carried out. The research aims to understand the effect of 5 % methanol and various concentrations of coconut milk (0 %, 2 %, 4 %, 6 %, 8 % and 10 %) on the motility of Koi fish spermatozoa 48 hours post-cryopreservation. Koi fish milt in this research was collected by stripping method and then evaluated microscopically and macroscopically. Results obtained are further assayed statistically using the Saphiro-Wilk normality test, Levene homogeneity test, one way Anova test, and Tukey multiple comparison test. One way Anova test showed that various concentration of coconut milk had significant difference (P < 0.05) of average value of motility on spermatozoa of Koi fish 48 hours post cryopreservation. A combination of 5 % methanol with 6 % coconut milk is the optimum concentration of cryoprotectant because it produced the highest average value of spermatozoa motility (79.13 ± 4.18 %).