Open AccessMultiwavelength confocal fiber optic microscopy
Author(s) -
J A Sosa-Acosta,
Celia SánchezPérez
Publication year - 2021
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1723/1/012020
Subject(s) - optics , confocal , confocal microscopy , materials science , microscope , pinhole (optics) , collimated light , microscopy , optical fiber , chromatic aberration , resolution (logic) , scanning confocal electron microscopy , chromatic scale , laser , physics , computer science , artificial intelligence
We present a multiwavelength fiber optic confocal microscope (MCFOM) based on the use of a wide spectral emission light source and some commercially available fiber optic components to make a reliable and compact system. We use single mode fiber optics SMF-28 to illuminate and collect the reflected light from the sample avoiding the use of a pinhole as a filter for the unfocused planes; as well as two lenses to collimate the light. The chromatic aberration produced by the lenses is used to interrogate several planes simultaneously, eliminating the confocal scanning movement obtaining an average confocal resolution of 4 μm and a surface scanning resolution of 1 μm. We demonstrated enough sensitivity to scan biological samples with a depth of penetration of 26 μm showing the potential use of this proposal in research laboratories as an alternative low cost confocal microscopy.