Open AccessVisualization of the intracellular structures of bacteria using expansion microscopy
Author(s) -
N. A. Rumyantseva,
A. D. Vedyaykin,
Innokentii E. Vishnyakov,
M. A. Khodorkovskii
Publication year - 2020
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1697/1/012048
Subject(s) - microscopy , resolution (logic) , visualization , ftsz , intracellular , microscope , super resolution microscopy , biological system , biophysics , dna , sample (material) , materials science , nanotechnology , computational biology , chemistry , computer science , bacteria , optics , bacterial protein , biology , physics , scanning confocal electron microscopy , artificial intelligence , chromatography , biochemistry , genetics
Expansion microscopy (ExM) is a powerful tool which allows to visualize intracellular structures with increased resolution due to physical widening of the sample. ExM resolution is comparable to that of some super-resolution microscopy techniques, but it does not require the use of complex and expensive optical equipment, thereby providing improvement in resolution even if relatively simple microscopes are used. In this work we successfully utilized ExM to visualize structures formed by FtsZ protein and DNA in Escherichia coli cells. The results of the work demonstrate that structures formed by both FtsZ and DNA are highly sensitive to the sample preparation procedure which should be optimized in each case of the ExM use.