Open AccessElastomer planar device for nucleic acids extraction
Author(s) -
N. N. Germash,
N. A. Esikova,
P. K. Afonicheva,
I. E. Antifeev,
D. G. Petrov,
A. A. Evstrapov
Publication year - 2020
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1697/1/012043
Subject(s) - nucleic acid , elastomer , extraction (chemistry) , materials science , microfluidics , soft lithography , modulus , composite material , chromatography , nanotechnology , chemistry , biochemistry , medicine , alternative medicine , pathology , fabrication
In this paper a device that provides the nucleic acids extraction and loading into a microfluidic chip for subsequent analysis by amplification methods was performed. The device is made by soft lithography from Silastic T4. The mechanical properties of the material (Young’s modulus) and the contact angle after Ar plasma treatment were studied. The strength of the connection of the device elements was evaluated. The device usability was checked by nucleic acids extraction from E.coli samples and using model solutions of the GAPDH gene cDNA.