Open AccessMultichamber hybrid microfluidic chips for nucleic acids detection by qPCR assay
Author(s) -
P. K. Afonicheva,
А. N. Zubik,
A. L. Bulyanitsa,
G. E. Rudnitskaya,
A. A. Evstrapov
Publication year - 2020
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1695/1/012060
Subject(s) - nucleic acid , microfluidics , silicon , chemistry , chromatography , nanotechnology , materials science , biological system , biology , optoelectronics , biochemistry
The efficiency of silicon-glass chips for the nucleic acids analysis with a concentration of 10 6 -10 2 copies/µl using various algorithms of searching threshold cycles is shown. Hypotheses that explain the observed PCR efficiency decrease for microfluidic chips compared to polymer test tubes are discussed.