Open AccessStudy of droplet formation regimes in a pressure control mode in microfluidic chip for screening cell libraries
Author(s) -
N. A. Filatov,
Anton Bukatin
Publication year - 2020
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1695/1/012053
Subject(s) - microfluidics , operability , flow control (data) , generator (circuit theory) , nanotechnology , materials science , controller (irrigation) , process engineering , flow (mathematics) , water in oil , computer science , engineering , mechanics , emulsion , power (physics) , chemical engineering , physics , telecommunications , software engineering , quantum mechanics , agronomy , biology
To create a high throughput system for screening single cells in “water-in-oil” droplets, a key role is played by the method of controlling fluid flows in a microfluidic droplet generator. This is due to the fact that it is required to obtain monodisperse drops-microcapsules, place cells in them and be able to manipulate them. For these purposes, not only systems based on syringe pumps are suitable, but also pressure control units may be used. Unfortunately, pressure control instruments on the market for researchers are represented by only a few companies and are quite expensive. In this work, we develop a homemade microfluidic pressure controller based on SMC ITV 0010 pneumatic regulators and show its operability. Also, we investigated the conditions for reproducible generation of water-in-oil emulsions in a microfluidic flow focusing droplet generator by pressure driven fluid flows.