Open AccessRPMI-1640 laden alginate hydrogel microcapsule produced by a coaxial electrohydrodynamic method
Author(s) -
Lu Zhang,
Tan Fulong,
Jia Ruiwen,
Yiwei Wang,
Zhihai Wang,
Xi Chen,
Jingang Gui
Publication year - 2020
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1676/1/012221
Subject(s) - electrohydrodynamics , coaxial , nozzle , materials science , electrode , volumetric flow rate , sodium alginate , voltage , microfluidics , nanotechnology , biomedical engineering , chemistry , electrical engineering , sodium , mechanical engineering , engineering , physics , quantum mechanics , metallurgy
The microencapsulation of biomaterials has a broad application prospects in the field of biomedicine. This paper introduces design and setup of a coaxial microcapsule generator based on electrohydrodynamic (EHD) method. It includes a liquid supply system, a coaxial nozzle, a collector electrode, and a high voltage power supply. The ejection process is studied via LED illumination and a high speed CMOS camera. For the shell and core materials, alginate and RPMI-1640 culture medium are applied respectively. The effects of electric voltage and flow rate on the ejection frequency and volume of micro-droplet are investigated. The generated micro-droplets react in the collector electrode with CaCl 2 solution. Alginate hydrogel microcapsules are produced with RPMI-1640 included. Good encapsulation is confirmed, by fluorescence microscopy, and with RPMI-1640 stained by sodium fluorescein. The produced microcapsule may be used in biological cell encapsulation, in the future.