Open AccessTerpenoid as Antibacterial Produced by Endophyte Fusarium oxysporum LBKURCC41 from Dahlia variabilis Tuber
Author(s) -
Finna Piska,
Hilwan Yuda Teruna,
Saryono
Publication year - 2020
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1655/1/012034
Subject(s) - fusarium oxysporum , dahlia , plant use of endophytic fungi in defense , fusarium , strain (injury) , fermentation , antibacterial activity , botany , endophyte , terpenoid , antimicrobial , chemistry , biology , food science , microbiology and biotechnology , bacteria , genetics , anatomy
Fusarium oxysporum LBKURCC41 is an endophytic isolated from Dahlia variabilis tubers from Padang Luar, West Sumatra. The strain was fermented using modified Huangs’ media, ie using corn as a carbon source for 15 days. The purpose of this study is to isolate secondary metabolites from LBKURCC41 strains. The filtrate from the liquid fermentation media was extracted using EtOAc:MeOH at a ratio of 95:5 to 50:50. Purification using fast chromatography was performed with a mixture of BAW (5:1:4). The structure was elucidated by, FTIR and 1 H NMR spectroscopy. The isolate was identified as a sesquiterpenoid, with weak antimicrobial activity against E.coli and S. aureus with 2.1 mm clear zone and concentration of 10 ppm.