Open AccessIsolation of Antibiotic-Producing Bacteria from Extreme Microhabitates in Mangrove Ecosystem
Author(s) -
Irwan Effendi,
Amries Rusli Tanjung,
Desy Mutia Sari
Publication year - 2020
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1655/1/012019
Subject(s) - bacteria , microbiology and biotechnology , biology , agarose gel electrophoresis , 16s ribosomal rna , bacillus amyloliquefaciens , bacillus cereus , strain (injury) , dna , biochemistry , genetics , anatomy
This study aims to isolate and identify the antibiotic-producing bacteria from extreme microhabitates in the marine ecosystem against fish pathogenic bacteria ( V. alginolyticus, A. hydrophila , dan Pseudomonas sp). Microbes from samples were grown on NA and TSA media. Well grown colonies were examined for their ability to produce antibiotics against the pathogens by the streaking and paperdisc diffusion method on MHA. Potential isolates were identified through a series of phenotypic and genotypic test. A number of 4 antibiotic-producing bacteria were selected. DNA of the bacteria were extracted using DNA easy mini column and amplified by using Kappa PCR kit with 16S rDNA 1A and 16S rDNA2A primers. The PCR results were analyzed on a 1.5% agarose gel in Tris-acetat-EDTA (TAE) electrophoresisuffer (4 mM Tris-acetate and 1 mM disodium EDTA at pH 8.0). The electrophoresis process was carried out at 60 mA (100-120 v) for 30-45 minutes read by UV Transilluminator. Isolate Ni 5 have a 99% homological similarity to Bacillus amyloliquefaciens strain SH20. Bacterial isolate Xy 3 has a 100% homological similarity with Bacillus cereus strain NY180. Isolate S 11 has a 97% homological similarity with Enterobacter hormaechei strain CGAPGPBBS-064. Isolate K 22 has a 99% homological resemblance to Klebsiella pneumoniae strain MW-W 754.