Open AccessOptimization of the Extraction Process of Anti-Vibrio parahaemolyticus Active Substances from Coptidis Rhizoma (Huanglian)
Author(s) -
Xintong Wang,
Siya Guo,
Zongyi Zhang,
Qian Xu,
Lei Guo
Publication year - 2020
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1637/1/012108
Subject(s) - vibrio parahaemolyticus , extraction (chemistry) , chromatography , response surface methodology , box–behnken design , methanol , solvent , chemistry , process (computing) , active compound , biology , computer science , stereochemistry , bacteria , biochemistry , genetics , organic chemistry , operating system
The purpose of this study is to optimize the extraction process of the anti-Vibrio parahaemolyticus active substances from Coptidis rhizoma (Huanglian). Single factor experiment, Box-Behnken experimental design combined with response surface methodology were used to optimize the extraction process. The obtained optimal extraction conditions followed as: extraction solvent is 100% methanol, extraction temperature of 86 °Q liquid/material ratio of 21 mL/g’ extraction time of 116 min. The actual value of the inhibition zone of the extract can reach 15.62±0.16 mm, which is not significantly different from the predicted value of 15.12 mm. This indicates that the above method is feasible to optimize the extraction process of the anti- V. parahaemolyticus active substances from Coptidis rhizoma.