Open AccessCellulolytic Fungi Isolation and Identification from Fermetoge, Cattle Feed Composed of Fermented Water Hyacinth (Eichhornia crassipes) And Corn (Zea mays) Cob
Author(s) -
ISNAWATI ISNAWATI,
NI’MATUZAHROH NI’MATUZAHROH,
Tini Surtiningsih
Publication year - 2020
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1569/4/042082
Subject(s) - hyacinth , cellulase , fermentation , starter , biology , cellulose , food science , eichhornia crassipes , isolation (microbiology) , aspergillus fumigatus , botany , microbiology and biotechnology , aquatic plant , biochemistry , paleontology , ecology , macrophyte
Fermetoge is fermented feed produced from fermentation of water hyacinth and corn cob mixture, commonly used as cattle feed in East Java, Indonesia. Fermetoge is composed of these cellulose-contained materials easily degraded by cellulase enzymes produced by fungi during fermentation. This study was aimed to isolate fungi from Fermetoge and screen their ability in degrading cellulose. Ten fungi isolates were found from fermetoge, three out of which indicated high cellulolytic activity. The three of them were identified based on partial ITS 1 and ITS 4 gene sequencing as Rhizophus stolonifer , Aspergillus fumigatus, and Penicillium sp. , with similarity level of 95%, 100%, and 89% respectively. Isolation and identification of fungi from fermetoge can give insight into cellulose-degrading fungi species to be studied for further developing starter culture and improving the quality of starter-base fermented feed.