Open AccessThe correlation between total antioxidant status with malondialdehyde and caspase 3 epididymal fluid type 2 diabetic rat and non diabetic
Author(s) -
Wulan Christijanti,
Achmad Zulfa Juniarto,
Lisyani Suromo
Publication year - 2020
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1567/3/032052
Subject(s) - malondialdehyde , thiobarbituric acid , medicine , antioxidant , endocrinology , streptozotocin , diabetes mellitus , trolox , caspase 3 , chemistry , antioxidant capacity , clinical chemistry , apoptosis , oxidative stress , lipid peroxidation , biochemistry , programmed cell death
The aim of this study is to determine the correlation between total antioxidant status (TAC) with malondialdehyde (MDA) and caspase 3. This study was divided into non-diabetic (n = 8) and type 2 diabetic rats (n = 8) induced by streptozotocin 65 mg / kg body weight. Epididymal fluid was examined after 28 days. Antioxidant status was examined by the trolox equivalent method, MDA with the thiobarbituric acid (TBA) reaction and caspase 3 expression with immunohistochemistry. Our results show that the mean of TAC in non-diabetic was significantly higher (P <0.000) than diabetic rats, MDA (P <0.000) and caspase 3 expression (P <0.001) in non-diabetic rats were significantly lower than diabetic rats. TAC was negative correlation with MDA (r = 0.881, P = 0.004) and caspase 3 (r = 0.898, P = 0.002) in the non-diabetic group. In the diabetic group, TAC was a negative correlation with MDA (r = 0.856, P = 0.007) and caspase 3 (r = 0.886, P = 0.003). In conclusion, total antioxidants have a negative correlation with MDA and caspase 3 expression.