Open AccessAnalysis of malondialdehyde and superoxide dismustase levels after exposure of electric cigarette in rats
Author(s) -
. Lisdiana,
W H Nugrahaningsih,
Inarotun Nufus
Publication year - 2020
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1567/3/032051
Subject(s) - malondialdehyde , superoxide dismutase , chemistry , nicotine , tbars , oxidative stress , lipid peroxidation , biochemistry , medicine
Electric cigarette (e-cigarette) is cigarette that operate on battery power to burn liquids and to produce a steam. One of the contents of an electric cigarette is nicotine. It is a chemical compound that can cause addiction and trigger oxidative stress. This study aims to analyze the levels of malondialdehyde and superoxide dismutase in the blood of rats that exposed to nicotine from e-cigarettes. The study was conducted on 30 male Wistar rats which divided into 5 groups, control group there are negative and positive and treatment group with nicotine 0,25 mg, 0,5 mg, 0,75mg with exposure to cigarette smoke for 30 days. Malondialdehyde and superoxide dismutase levels measurements using the TBARs method, the results that can be known using a spectrophotometer with a wavelength of 532 nm. SOD levels were measured by the calorimetry method. The One Way Anova analysis showed that malondialdehyde and superoxide dismutase levels in the control group were significantly different from all groups. The conclusion is that nicotine has an effect on increasing malondialdehyde and decreasing superoxide dismutase levels.