Open AccessStudy on UPLC Fingerprint of Lophatherum Gracile Brongn
Author(s) -
Xiaosong Yang,
Juan Kong,
Lingling Zhang,
Xiangpei Wang,
Xin He,
Hongmei Wu
Publication year - 2020
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1549/4/042003
Subject(s) - chromatography , fingerprint (computing) , chemistry , high performance liquid chromatography , phosphoric acid , gradient elution , artificial intelligence , computer science , organic chemistry
Objective: To establish UPLC fingerprint of Lophatherum gracile Brongn and provide reference for quality evaluation of Lophatherum gracile Brongn. Method: Agilent ZORBAX Eclipse Plus C 18 (2.1mm × 100mm, 1.8 μm) chromatographic column, Mobile phase acetonitrile-0.1% phosphoric acid water, gradient elution, flow rate 0.1 mL·min-1, column temperature 30°C and injection volume 1μL. Results: There are 20 common peaks in the chromatographic fingerprint of 10 batches of Lophatherum gracile Brongn at 270 nm, and the similarity is between 0.890-0.974. Conclusion: The UPLC fingerprint method established in this study is efficient, fast, specific and characteristic, and the sample information is rich, which can provide reference for the quality evaluation of Lophatherum gracile Brongn.