Open AccessSequence Analysis of BocAOP2.3 Gene in Cabbage
Author(s) -
Yuan Qiao,
Wenli Huang,
Yue Jian,
Hao Zheng,
Min Jiang,
Fen Zhang,
Bo Sun
Publication year - 2020
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1549/3/032035
Subject(s) - brassica , brassica rapa , gene , biology , open reading frame , isoelectric point , peptide sequence , valine , sequence analysis , capitata , botany , amino acid , genetics , brassica oleracea , biochemistry , enzyme
The AOP2.3 gene is an important gene affecting the side chain modification of aliphatic glucosinolate. Here, the Brassica oleracea var. capitata AOP2.3 (BocAOP2.3) gene sequence was obtained from the Brassica database (BRAD) and subjected to sequence analysis. It is indicated that the BocAOP2.3 gene contains a 936 bp open reading frame (ORF) encoding a protein of 311 amino acids with a calculated molecular weight of 33.62 kD, the isoelectric point (pI) is 4.46. The highest number of amino acids is Valine (Val), whereas the lowest number is Tryptophan (Trp). Its prediction formula is C 1455 H 2295 N 397 O 505 S 6 . Its total hydrophobicity index is -0.5, the solution instability index is 29.25, and the fat solubility index is 77.46. Sequence alignment indicated that the BocAOP2.3 protein is more closely related to Brassica napus and Brassica Rapa , those all belonged to the Cruciferous genus. The results of this study provide a molecular basis for elucidating the function of BocAOP2.3 gene in cabbage.