Open AccessSubcellular Localization of BoaZDS Gene in Chinese kale
Author(s) -
Yuan Qiao,
Wenli Huang,
Yue Jian,
Hao Zheng,
Min Jiang,
Jiaqi Chang,
Fen Zhang,
Bo Sun
Publication year - 2020
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1549/3/032033
Subject(s) - subcellular localization , gene , protoplast , plasmid , chloroplast , biology , function (biology) , microbiology and biotechnology , genetics , biochemistry
The experiment used the ‘Sijicutiao’ mustard sterile seedlings as the material, firstly constructed the BoaZDS subcellular localization vector with the cloned BoaZDS gene, and extracted the plasmid, then separated the Chinese kale leaf protoplast by enzymatic hydrolysis. After purification, the BoaZDS gene was transiently transformed, and then the position of the BoaZDS gene was observed under a fluorescence microscope. The results showed that the BoaZDS gene was localized in the chloroplast, which was consistent with the predicted results of bioinformatics software. The results provide a basis for studying the function of the ZDS gene in Chinese kale.