Open AccessIsolation And Characterization Of Flavonoid From Leaves Of Bauhinia kockiana Lour And Antibacterial Activity
Author(s) -
Amelia Gresya Pasaribu,
Kiki Nurtjahja
Publication year - 2020
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1542/1/012045
Subject(s) - flavonoid , ethyl acetate , antibacterial activity , chemistry , chloroform , chromatography , methanol , column chromatography , agar diffusion test , nuclear chemistry , bacteria , organic chemistry , biology , genetics , antioxidant
The Isolation and purification of flavonoid compounds from the leaves of Bauhinia kockiana Lour had been done. Concentrated methanol extract was hydrolyzed with HCl 6%, partitioned with chloroform and it was separated by column chromatography using n-hexane: ethyl acetate (60:40 v/v). Pure yellow-crystalline amorphous compound; mass = 33.8 mg, m.p = 155 °C and Rf = 0.29, was then characterized by spectroscopy UV-Visible, FT-IR and 1 H-NMR. Interpretation of Spectrofotometer of compound compared with data standard showed the compound is flavonoid Flavonol. The antibacterial activity test toward methanol, hexane and ethyl acetate extracts respectively was conducted using diffusion method which inoculated bacteria were dipped into Mueller Hinton Agar. Results showed that inhibitory effect of bacterial growth was better with effective inhibition zone againsts Escherichia coli at concentration 500 mg/ml with diameter zone 17.8 mm