Open AccessSynthesis of colloidal silver nanoparticles and their bactericidal effects on E. coli, S. epidermidis and oral plaque
Author(s) -
José Ernesto Ramírez,
Juliana Ortiz,
Jorge Andrés Cuellar,
Carlos Andrés Naranjo,
Francy Nelly Jiménez,
Oscar Moscoso Londoño
Publication year - 2020
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1541/1/012017
Subject(s) - surface plasmon resonance , silver nanoparticle , silver nitrate , staphylococcus epidermidis , nanoparticle , ascorbic acid , ethylene glycol , nuclear chemistry , transmission electron microscopy , chemistry , colloid , absorption spectroscopy , materials science , nanotechnology , bacteria , staphylococcus aureus , optics , organic chemistry , biology , genetics , food science , physics
Silver nanoparticles have been obtained by colloidal synthesis using two different reducing agents: ascorbic acid and ethylene glycol. The colloids have been characterized by UV-Vis Spectroscopy, atomic absorption and transmission electron microscopy (TEM). The UV-visible spectra show the typical peak with a maximum ranging between 390-420 nm, due to the plasmon resonance of spherical silver nanoparticles. TEM micrographs show non-aggregated spherical silver nanoparticles whit diameters between 30 and 50 nm. The antibacterial effect was tested in three different bacteria cultures: Escherichia coli (gram negative) , Staphylococcus epidermidis (gram positive) and dental plaque, which were grown in Mueller-Hinton agar. For comparative purposes the bactericidal effect of silver nitrate was also evaluated. Inhibition index ( IIC ) was calculated, obtaining satisfactory results for the three kinds of microorganism when silver nanoparticles are used.