Open AccessStudy on the Expression and Significance of P16 Gene Methylation Based on Information Technology Statistics in Female Cervical Cancer in Hunan Povince
Author(s) -
Jingbo Zhou,
Wei Jin
Publication year - 2020
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1533/2/022073
Subject(s) - methylation , exon , cpg site , cervical cancer , biology , gene , dna methylation , immunohistochemistry , cancer research , gene expression , cancer , pathological , microbiology and biotechnology , bisulfite sequencing , pathology , genetics , medicine , immunology
To explore the significance of p16 gene methylation and abnormal expression in cervical cancer, Methylation specific PCR (MSP) was used to detect 5 ′ CpG island methylation of p16 gene promoter in 60 cervical cancer tissues of different pathological types and clinical stages, who was chosen in Human province. Detection of homozygous deletion of exon 1 (E1) and exon 2 (E2) of p16 gene by PCR. Immunohistochemical analysis of p16 protein expression. The results showed that there was no methylation of p16 gene in normal control tissues and paracancerous tissues and no E1 and E2 deletions and abnormal p16 protein expression were found. The methylation rate of 60 cervical cancer samples was 21.67% (13 / 60), the deletion rate of p16 gene was 15.00% (9 / 60), and the expression rate of p16 protein was 51.67% (31 / 60). It can be seen that the positive expression rate of p16 gene protein decreased with the increase of clinical stage. The results suggest that p16 gene inactivation is common in cervical cancer and closely related to the pathological grade. p16 gene methylation plays a role in the development of cervical cancer.