Open AccessExtraction of Polyhydroxyakanoate (PHA) from Palm Oil Mill Effluent (POME) using Chemical Solvent Extraction
Author(s) -
H. L. Shakirah,
Z. N. A. Zulilah,
M. S. N. Aniyyah,
A. Nabihah,
G. S. Radhiah
Publication year - 2020
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1532/1/012015
Subject(s) - pome , acetone , solvent , extraction (chemistry) , methyl isobutyl ketone , chemistry , ethyl acetate , chromatography , effluent , polyhydroxyalkanoates , fermentation , pulp and paper industry , organic chemistry , waste management , biology , bacteria , engineering , genetics
Palm oil mill effluent or POME is the waste water discharge from palm oil milling activities. Polyhydroxyalkanoate or PHA is the linear polyester that produces by bacterial fermentation of sugar or lipids with nature process. The objectives of this research are to extract PHA from POME using chemical solvent and to study the percentage of purity and recovery of PHA. A methodology was developed for PHA extraction from palm oil mill effluent using chemical solvent. PHA from POME can be extracted by using chemical extraction method by using non halogenated solvent that are acetone, methyl isobutyl ketone and ethyl acetate. The result show percentage of mass PHA recovery with acetone has high percentage that is 93% follow by ethyl acetate with 81.2% and lastly with methyl isobutyl ketone with 66%. Meanwhile, the PHA purity percentage by using acetone is 71%, follow by ethyl acetate 58% of purity obtain lastly methyl isobutyl ketone with 49% PHA purity obtain. PHA can be extracted from POME using chemical solvent extraction method.