Open AccessPhotodynamic Inactivation of Streptococcus mutan Bacteri with Photosensitizer Moringa oleifera Activated by Light Emitting Diode (LED)
Author(s) -
Aulia Iwinda Mardianto,
Ernie Maduratna Setiawatie,
Winda Puji Lestari,
Ahmed Rasheed,
Suryani Dyah Astuti
Publication year - 2020
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1505/1/012061
Subject(s) - photosensitizer , photodynamic therapy , chemistry , moringa , bacteria , photochemistry , food science , biology , organic chemistry , genetics
A Photodynamic Inactivation (PDI) research has been done as a method of photodynamic in a medical treatment that combine light with photosensitizer. The light energy absorption by the photosensitizer molecules will produce reactive oxygen species that cause biological damage. Photoinactivation has done by combining blue LED light and exogenous porphyrin photosensitizer Moringa oleifera leaf extract to a major cause of dental caries disease, Streptococcus mutan . The goal of this research is to analize a maximum potential of LED exposure angle to inactivates a Streptococcus mutan bacteria added by 20% concentration of Moringa oleifera leaf extract photosensitizer. The wavelength of blue LED light used is 450.00 ± 0.21 nm in 100% Pulse Width Modulation (PWM). Total Plate Count (TPC) is used as a method to determine the loss of bacteria viability in unit CFU/ml. The stastical test result show an angle that potentially inactivate the bacteria by the exposure of two groups treatment with six variation of angle and an exposure time is 90° angle which has different meaning with another variation (p < a = 0.05), that means the best angle to use is perpendicular which has a percentage result of absorbent media for 180 second duration is (30,301 ± 4,231)% of bacterial death without photosensitizer and increased to (46,742 ± 1,667)% bacterial death with a photosensitizer.