Open AccessApplication of High Performance Liquid Chromatography Method for Triadimenol Analysis in Water Sample
Author(s) -
Dadan Hermawan,
Cacu,
Suwandri,
Amin Fatoni,
Mudasir Mudasir,
Hassan Y. AboulEnein
Publication year - 2020
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1494/1/012032
Subject(s) - chromatography , high performance liquid chromatography , detection limit , reproducibility , linear range , chemistry , calibration curve , acetonitrile , extraction (chemistry) , sample preparation , analytical chemistry (journal)
Triadimenol is the most important triazole fungicide used for prevention and treatment of plant fungal disease. A simple high performance liquid chromatography (HPLC) method for triadimenol analysis in water sample is reported in this study. The optimized HPLC system using C 18 column was achieved using mobile phase composition containing acetonitrile-water (70:30, v/v), a flow rate of 1.0 mL/min, UV detection at 220 nm, and analysis time within 2.5 min. The calibration graph was linear in the range 5 to 50 mg/L with r 2 of 0.9997. The limit of detection (LOD) and limit of quantification (LOQ) obtained were 1.23 and 4.12 mg/L, respectively. Combination of solid phase extraction (SPE) and the optimized HPLC method has been successfully applied for the determination of triadimenol in water sample with a recovery of 94.24 % (RSD = 5.38%, n = 4). The proposed HPLC method provides short analysis time, high reproducibility and high sensitivity.