Open AccessOptimization of complex NH3 with Cu2+ ions to determine levels of ammonia by UV-Vis spectrophotometer
Author(s) -
Desy Guspita,
Alizar Ulianas
Publication year - 2020
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1481/1/012040
Subject(s) - ammonia , absorbance , spectrophotometry , chemistry , ion , copper , color reaction , inorganic chemistry , nuclear chemistry , analytical chemistry (journal) , chromatography , organic chemistry , reagent
Ammonia analysis based on complexing ammonia with Cu 2 + ions by UV-Vis spectrophotometry. This research aims to obtain uptake at the maximum wavelength of the copper-ammonia complex, optimum conditions and validation of UV-Vis spectrophotometry. The reaction between Cu 2+ ions which are blue with ammonia colorless occur in solution to form complex compounds [Cu(NH3)4] 2 + dark blue, the maximum absorbance is obtained at a wavelength of 615 nm. Optimization of the ammonia complexing the Cu 2 + ions provide optimum conditions a solution of Cu 2 + concentrations of 0.01 M in ammonia concentration of 0.04 M, pH 7, and the optimum time to form a complex occurred in the 30th minute and complex compounds can be stable for 90 minutes (± 1 hours 30 minutes). The analytical method validation ammonia using Cu 2+ ions provide linear regression equation y = 4,772 x + 0.333 with R 2 = 0.989 ammonia concentration range of 0.003 M to 0.08 M; LOD 0.01 M; LOQ 0.04 M; % RSD = 1.32%; and% recovery = 102.03%. The concentration of ammonia in the wastewater sample application obtained the ammonia concentration of 0.04 M. Based on the results of the validation, Ammonia solution with Cu 2+ ion as a complex used for the determination of ammonia levels by UV-Vis spectrophotometry.