Open AccessUnripe Papaya (Carica papaya L.) Aqueous Extract (UPAE) for the acute toxicity test on fatty liver changes
Author(s) -
Yuktiana Kharisma,
Meike Rachmawati,
R. Anita Indriyanti,
Titik Respati
Publication year - 2020
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1469/1/012013
Subject(s) - carica , fatty liver , steatosis , steatohepatitis , ingestion , toxicity , phytochemical , oral administration , medicine , dose , fibrosis , haematoxylin , histopathology , traditional medicine , pharmacology , pathology , biology , staining , botany , disease
We investigated whether the oral ingestion of aqueous extract of unripe papaya fruit (UPAE), would also induce fatty liver changes by analysing the UPAE-treated mice qualitatively. This study used laboratory experiments which performed by a proposed (new) recommended method with 11 subjects of female mice which were administered with single oral doses of 0; 50; 200; 400; 800; 1, 000; 1, 500; 2, 000; 3, 000; 4, 000; 5, 000 mg/kg respectively. All these mice were euthanized after 24 hours of UPAE administration. Histopathological studies were conducted using liver samples which were stained by Haematoxylin-Eosin (HE). The UPAE did not cause death in all doses but did induce hepatic steatosis and steatohepatitis at 4, 000 and 5, 000 mg/kg dosages. Ballooning degeneration, necroinflammation and portal fibrosis revealed at 200 – 5, 000 mg/kg. These findings suggest that the UPAE provoked the fatty liver changes at the highest dose administration. It may be obtained from a multiple hit process by the accumulation of the phytochemical compounds of UPAE.