Open AccessStaining protocols for whole-body and fast tissue clearance for marine invertebrates
Author(s) -
E. P. Kotsyuba,
Olga V. Yurchenko,
Dmitrii Kamenev,
A. Savelieva,
Alexander V. Kalachev,
V. A. Milichko,
Vyacheslav Dyachuk
Publication year - 2020
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1461/1/012105
Subject(s) - marine invertebrates , invertebrate , immunostaining , biology , anatomy , ecology , immunohistochemistry , immunology
Marine invertebrates are the most morphologically varied group of animals with tough and smooth covers, external and internal organ structures and different body designs in general. Very often researchers cannot see internal deep structures without mechanical damage of animals. Here, we focus on “not damage” protocols of immunostaining and clearance of whole-body of invertebrates.