Open AccessThe effect of siam orange (Citrus nobilis Lour.) and pineapple (Ananas comosus (L.) Merr.) juices combination in extender on Garut Ram (Ovis aries L.) spermatozoa quality post-cryopreservation
Author(s) -
Huriyah Adani Saoemi,
Ekayanti Mulyawati Kaiin,
S Setiorini,
Muhammad Gunawan
Publication year - 2020
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1442/1/012066
Subject(s) - extender , ananas , orange (colour) , cryopreservation , cryoprotectant , biology , semen , citrus × sinensis , chemistry , zoology , food science , botany , anatomy , embryo , organic chemistry , polyurethane , microbiology and biotechnology
The research aimed to find out the effect of various concentrations of siam orange (Citrus nobilis Lour.) and pineapple (Ananas comosus (L.) Merr.) juices combination (1:1) in extender on garut ram (Ovis aries L.) spermatozoa quality 24 hours post-cryopreservation. Semen samples were collected from five Garut Rams once a week using an artificial vagina. The samples were diluted in Tris-egg yolk extender with siam orange and pineapple juices combination 0 %, 5 %, 10 %, 15 %, and 20 %. Diluted semen samples were loaded into mini straws (0.25 mL) with 50 million cells/ml dosage. Samples were equilibrated at 5°C for two hours, then frozen and stored in liquid nitrogen. The one factor ANOVA and continued with Duncan test showed a significant differences (P<0.05) between control and sample with 10 % juices combination in the percentage of spermatozoa motility and membrane integrity, a significant difference (P<0.05) between control and sample with 5 % along with 10 % juices combination in the percentage of spermatozoa viability. Siam orange and pineapple juices combination 10% in extender was able to minimize the reduction of spermatozoa quality post-cryopreservation based on the percentage of spermatozoa motility (68.06 ± 2.34 %), viability (41.47 ± 6.64 %) and membrane integrity (46.00 ± 5.65 %).