Open AccessNon-fecal and fecal coliform tests of ready-to-eat food and drinks using fluorogenic and chromogenic media
Author(s) -
M R Naratama,
Iman Santoso
Publication year - 2020
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1442/1/012064
Subject(s) - fecal coliform , chromogenic , escherichia coli , feces , food science , coliform bacteria , agar , microbiology and biotechnology , biology , chemistry , bacteria , water quality , chromatography , ecology , biochemistry , genetics , gene
The aim of this research was to assess the microbiological quality of ready-to-eat foods and drinks. Fifteen samples were enriched in buffered peptone water (BPW) before tested for the presence of coliforms using two chromogenic media (Chromocult Coliform Agar-Enhanced Selectivity (CCA-ES) and Harlequin E. coli agar (HEC)) and a fluorogenic medium (Readycult Coliform 100 (RC 100)) at 37°C. Results showed that all samples contained non-Escherichia. coli coliforms and 12 of them contained Escherichia coli . Twelve E. coli strains and 15 non-E. coli coliform isolates were isolated. Fecal coliform tests showed that the isolated E. coli from 12 samples were of fecal origin while only 7 out of 15 coliform isolates were fecal non-E. coli coliforms.