Open AccessLED illumination module for fluorescence and spectroscopic studies in microscopy
Author(s) -
V. I. Batshev,
A. A. Blokhin,
Artyom Belov
Publication year - 2019
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1421/1/012036
Subject(s) - dichroic glass , dichroic filter , optics , fluorescence , light emitting diode , optoelectronics , materials science , wavelength , radiation , microscopy , diode , physics
In this report we produce a microscopic illuminator consisting of four light emitting diodes (LEDs) with different wavelength. The radiation power of each LED could be controlled independently. The dichroic mirrors are used as a beam combiner. So, it is possible to create a highlight of the required spectral composition on the object under study. This illuminator is useful both for fluorescent analysis or spectral imaging.