Open AccessFluorescent detection upon droplet amplification of nucleic acids
Author(s) -
A. N. Tupik,
A. L. Bulyanitsa,
G. E. Rudnitskaya,
Y. S. Posmitnaya,
Д. А. Варламов,
A. A. Evstrapov
Publication year - 2019
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1410/1/012079
Subject(s) - nucleic acid , microfluidics , emulsion , fluorescence , nucleic acid detection , chemistry , biological system , sensitivity (control systems) , nanotechnology , chromatography , materials science , biology , biochemistry , physics , engineering , quantum mechanics , electronic engineering
Droplet microfluidics with amplification of nucleic acids allows to achieve sensitivity at the level of single molecules and is in demand in medical diagnostics, forensic science, and genetic research. The results of detection of nucleic acid amplification in liquid and water-in-oil emulsion are compared on the example of model solutions. Since the results of quantitative analysis are based on the calculation of the proportion of drops with a positive reaction result, one of the tasks was to study the features of automatic counting of drops in different zones of the reaction chamber.