Open AccessVirus-assembled technology for next generation bioenergy harvesting devices
Author(s) -
Sangwook Chu,
Amanda Brown,
James N. Culver,
Reza Ghodssi
Publication year - 2019
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1407/1/012029
Subject(s) - glucose oxidase , electrode , bioconjugation , electrochemistry , nanotechnology , tobacco mosaic virus , electron transfer , chemistry , materials science , biosensor , catalysis , chemical engineering , biochemistry , virus , photochemistry , virology , engineering , biology
This work reports Tobacco mosaic virus (TMV)-based assembly of glucose oxidase (GOx) electrodes for the development of advanced enzymatic biofuel cells (EBCs). Cysteine-modified TMV (TMV1cys), self-assembled on Au electrodes, has served as a template for augmenting the GOx immobilization density via a robust chemical conjugation route. Enhanced enzymatic activity of the TMV1cys/GOx electrodes have been confirmed via colorimetric assay, and the electrochemical transduction of the catalytic reactions via mediated electron transfer resulted in a ~25-fold increase in electrochemical current compared to previous work using a similar strategy. This enhancement is attributed to the on-chip bioconjugation strategy presented in this work, leveraging the robust and high density self-assembly of TMV1cys on Au surface. The performance of an EBC consisting of TMV-assembled enzyme electrodes is reported for the first time, generating a maximum power density of 860 nW/cm 2 .