Open AccessThe experimental chemical hardness in the interaction between β-tubulin and epothilone B
Author(s) -
Sandra P. Corzo,
Paulo Agenor Alves Bueno,
David A. Miranda
Publication year - 2019
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1403/1/012016
Subject(s) - epothilone , tubulin , chemistry , stereochemistry , molecule , logarithm , microtubule , materials science , biology , mathematics , organic chemistry , mathematical analysis , microbiology and biotechnology
We showed the use of the experimental chemical hardness in the analysis of the interaction between β -tubulin and epothilone B. The interaction is analyzed by exposing the Epothilone B to a sensor based on an organic self-assembled monolayer functionalized with Beta tubulin. The proposed methodology is nondestructive and label-free. It allows us to compare the interaction between Beta-tubulin and different concentrations of the pharmacological molecule, i.e ., epothilone B. The interaction is analyzed by the experimental hardness obtained according to the Miranda-Bueno methodology. We observed a linear behavior between the logarithm of the chemical hardness and the logarithm of epothilone B concentration. The results suggest the experimental chemical hardness can be used to compare the interaction of β -tubulin with epothilone B and with other pharmacological molecules.