Open AccessStudy of the processes of cleaning biological and chemical substances from implants of biological origin using supercritical CO2
Author(s) -
И. В. Кузнецова,
N. S. Sandugey,
И. М. Гильмутдинов,
И. И. Гильмутдинов,
A. N. Sabirzyanov
Publication year - 2019
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1399/2/022038
Subject(s) - supercritical fluid , supercritical carbon dioxide , reagent , chemistry , solvent , scanning electron microscope , chromatography , matrix (chemical analysis) , penetration (warfare) , chemical engineering , materials science , organic chemistry , composite material , operations research , engineering
Chemical reagents used in standard technologies are significantly inferior in effectiveness to their intended use in the purification of a biological matrix by supercritical carbon dioxide due to several reasons: retention of residual solvent in the structure of the biological matrix, the presence of pronounced cytotoxicity, problems associated with capillary effects (limited penetration of the reagent to the cleaned surface), pronounced allergenicity of chemical reagents. Laboratory studies of the influence of pressure processing from 20 to 35 MPa of a two-stage bone matrix cleaning process at a holding temperature of 308, 313 K to the 60-minute static processing time and the 240-minute dynamic processing time were carried out, including subsequent biochemical analyzes to determine residual DNA, calcium protein in the obtained samples. The addition of a cosolvent - ethanol in an amount of 5% of the main solvent of supercritical CO 2 was studied. Pyrogenic testing was carried out. All samples are recognized not pyrogenic. Physical properties and surface morphology were determined using a Carl Zeiss AURIGA CrossBeam scanning electron microscope. Identification and quantification of potential degradation products using liquid chromatography was determined.