Open AccessGenomic Profile of rIR and rIRR in Type 2 Diabetes Mellitus Rat Model toward Effect of Goat Milk CSN1S2 Protein
Author(s) -
Sonia Tambunan,
Hazoor Meidinna,
Rista Nikmatu Rohmah,
F. Fatchiyah
Publication year - 2019
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1374/1/012046
Subject(s) - biology , diabetes mellitus , gene , exon , genomic dna , type 2 diabetes mellitus , crossbreed , endocrinology , genetics
Diabetes mellitus is metabolic disorder with hypergycemia condition. Diabetes meilitus is commonly divided into two types, type 1 diabetes mellitus (T1DM) and type 2 diabetes mellitus (T2DM). The research focus was to determine the effect of CSN1S2 protein from Etawah Crossbred goat milk on DNA sequence of rIR exon 17 and rIRR exon 2 in T2DM rat model. The experimental rats were divided into four main groups, namely control group, diabetes group, control with CSN1S2 protein treatment group, and diabetes with CSN1S2 protein treatment group. We used 375, 750 and 1500 mg/kg BW of goat milk CSN1S2 protein for the treatment. The DNA was extracted from the rat liver, then amplified with a spesific primer of rIR and rIRR genes. The results of the amplification will be sequenced by using ABI 3730xl DNA Sequencer. The CSN1S2 protein treatment in rIR gene exon 17 did not affect a specific DNA sequence alteration. The diabetic rats treated with 375, 750 and 1500 mg/kg BW of CSN1S2 protein had the nucleotide changed to normal (3950A→3950G). Therefore, the CSN1S2 protein may have the function in triggering the DNA repair system.