The Effects of Isolate of Goat Casein Yoghurt Milk to MDA Level and Gastric Histopathology of Rats Exposed to 2,3,7,8-Tetrachlorinedibenzo-P-Dioxin (TCDD)

Dioxins are toxic compounds belonging to organochlorine compounds are highly reactive and produce free radicals that can damage stomach cells. 2,3,7,8- Tetrachlorinedibenzo-p-dioxin (TCDD) is the type of dioxin that has the highest toxicity. Casein goat milk yogurt has the potential as an antioxidant. This study aims to determine the preventive effect of goat milk yoghurt on exposure to dioxin observed levels of malondialdehyde (MDA) and gastric histopathology. Experimental research using Completely Randomized Design (RAL). The white rats ( Rattusnorvegicus ) were divided into 6 groups, the normal group, casein control (goat milk yoghurt dose 600 mg / kg BW), positive control (TCDD dose 100 ng / kg BW), treatment 1 (goat milk yoghurt 300 mg / kg BW and TCDD 100 ng / kg BW), treatment 2 (goat milk yoghurt 600 mg / kg BW and TCDD 100 ng / kg BW), and treatment 3 (goat milk yoghurt 900 mg / kg BW and TCDD 100 ng / kg BW). Gastric MDA levels were measured using the Thiobarbituric Acid (TBA) assay method with 530 nm wavelength UV-Vis spectrophotometric measurement and gastric histopathology using Hematoxylin-Eosin (HE) staining observed in the form of gastric mucosal cell damage. Gastric MDA levels were analyzed using one way ANOVA statistical test, α = 5% and gastric histopathology analysis were descriptively processed. The results showed that goat milk yoghurt with dose of 600 and 900 mg / kgBW was the best dose in preventing the increase of MDA level of gastric from white rat ( Rattusnorvegicus ) exposed TCDD significantly (p <0,01), and dose 900mg / kgBB prevented erosion of gastric mucosal cells. The conclusion of this research is giving goat milk goat yoghurt able to prevent the increase of MDA level and erosion of gastric mucosal cells caused by TCDD.