Open AccessSilane treatment of coated carbonate apatite scaffold affects bioactivity and cell viability
Author(s) -
Normahira Mamat,
Mariatti Jaafar,
Zuratul Ain Abdul Hamid,
Badrul Hisham Yahaya
Publication year - 2019
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1372/1/012054
Subject(s) - apatite , silane , scaffold , biocompatibility , viability assay , chemical engineering , materials science , surface modification , coating , chitosan , chemistry , nanotechnology , mineralogy , composite material , biomedical engineering , in vitro , organic chemistry , metallurgy , biochemistry , engineering , medicine
The aim of this study is to determine effects of surface treatment on coated-carbonate apatite (CO 3 Ap) scaffold using silane coupling agent of 3-(trimethoxysilyl) propyl methacrylate. The effect of coating properties with and without surface treatment were investigated through in vitro bioactivity and biocompatibility. Firstly, CO3Ap scaffold was fabricated by transforming β-TCP scaffold via hydrothermal treatment. CO 3 Ap scaffolds were treated in 2% silane solution before coating in chitosan solution (0.5%, 1% and 2%) by dipping method. The coated scaffold without silane treatment denoted as UTR while with silane treatment denoted as TR. It was found that, the presence of Si-OH group enhanced apatite growth on the coated scaffold. TR0.5 has capability to induce apatite growth for 7 days immersion in HBSS compared to UTR0.5. Rougher surface and different surface charge properties have influenced cell viability on the coated CO 3 Ap scaffold. To be concluded, it is important to note that bioactivity and cell activity were forced by surface energy or topography, composition and electrostatic interaction.