Open AccessIsolation, characterization and anticancer potential test of crude extract of L-asparaginase enzyme from siam weed leaf (Chromolaena odorata Linn): a novel source
Author(s) -
Yusriadi Yusriadi,
Aminah Ahmad,
N. Khaerah,
Rugaiyah Arfah,
Abdul Karim,
Harningsih Karim
Publication year - 2019
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1341/3/032016
Subject(s) - chromolaena odorata , weed , brine shrimp , enzyme , enzyme assay , biology , asparaginase , chemistry , traditional medicine , botany , biochemistry , medicine , ecology , lymphoblastic leukemia , genetics , leukemia
L-asparaginase enzyme is potentially isolated from Siam weed ( C. odorata L. ) and also used as anticancer treatment. The point of this study is to isolate crude extract of L-asparaginase as well as its characteristics for anticancer. The activity of the enzyme was observed by Nessler Method and its potential for anticancer was tested by Brine Shrimp Lethality Test (BSLT) method. The results showed the specific activity by 4.8234 UI/mg. The enzyme worked maximally at pH of 8, 37 °C for 30 minutes with Na + and K + being activator and Ca 2+ , Zn +2 , Mg +2 , Cu +2 , Co +2 , Mn +2 as inhibitor. Base on BSLT revealed an LC 50 value of 5.8063 μg/mL and proved that the enzyme has biotoxicity in high level. The results of this study denoted that C. odorata L. leaves able to be isolated and characterized by L-asparaginase enzyme and these reveals the potential to be developed as an anticancer in the future.