Open AccessFatty Acid Analysis of Marine Microalgae Chlorella vulgaris in Modified Medium Used GC-FID
Author(s) -
Dadang Kurnia,
Anne Yuliantini,
I. S. Cendana,
Zeily Nurachman
Publication year - 2019
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1338/1/012007
Subject(s) - chlorella vulgaris , fatty acid , chemistry , food science , fertilizer , fatty acid methyl ester , methanol , eicosapentaenoic acid , capric acid , organic chemistry , chromatography , botany , polyunsaturated fatty acid , biology , algae , catalysis , biodiesel , lauric acid
Microalgae oil can replace the role of fish and other vegetable oils. One of them is lipid in microalgae Chlorella vulgaris . This study aims to determine the composition of fatty acids in marine microalgae Chlorella vulgaris grown in fertilizer medium. Cultivation was carried out at room temperature, photoperiode 12:12 (dark: light); light intensity 10.000 lux and aerated for 24 hour. The optimum medium used was medium GMF fertilizer with concentration 10% (w/v). Lipid extraction used Blight-Dryer method with methanol, chloroform and aquadest as solvent. The lipid obtained was esterified by NaOH in methanol and H 2 SO 4 as a catalyst for 12 hours and analyzed used GC-FID with capric acid as a standard. The analysis result shows that was 18 fatty acid methyl ester compounds. The highest known fatty acid methyl esters compositions was 61.52% methyl stearate; 15.52% methyl palmitate; 4,69% methyl eicosanoate and 3.34% methyl heptadecanoate from total fatty acids. The unsaturated fatty acids identified were 0.17%. EPA (eicosapentaenoic acid) also 0.23% omega 3 and omega 6. Microalgae grown in a fertilizer medium produce quite high lipids. Although the levels of unsaturated fatty acids are still low, it does not rule out the possibility of being increased.