Open AccessScreening of Marine Microalgae Collected from Wakatobi as Anti-Tyrosinase
Author(s) -
Sentot Joko Raharjo,
V. N. Fatanah,
Dwi Susilaningsih,
Ma’ruf Kasim,
P. E. Susilawati,
Muzuni,
Delicia Yunita Rahman,
Tiến
Publication year - 2019
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1338/1/012003
Subject(s) - biomass (ecology) , diethyl ether , ethanol , metabolite , hexane , extraction (chemistry) , ethyl acetate , solvent , biology , chemistry , botany , horticulture , chromatography , food science , biochemistry , agronomy
Three microalgae isolates from Wakatobi waters collected by LIPI have been cultivated, ie LIPI 13-2-AL018, LIPI 13-3-AL066, and LIPI 13-2-AL072. Furthermore, the isolates were grown to create a growth curve, this is to determine the harvest time of biomass. Each isolate had different biomass harvest times: LIPI 13-2-AL018 and LIPI 13-2-AL072 isolates were harvested on the 14 th day, while LIPI 13-2-AL066 on day 12. After harvesting, the biomass was dried, destroyed the cell wall with sonicator. Extraction of secondary metabolite compounds was performed using three types of solvents, namely: ethanol, ethanol + n-hexane (1: 1), and diethyl ether. Based on the results of inhibition testing of tyrosinase it was found that the biggest inhibition was derived from extract of LIPI-13-2-AL066 isolate, using ethanol-hexane solvent.