Difference in Characteristic of Concentrate Powder of Corn (Zea mays var. indentata) Fermented by Bifidobacterium brevis as Natural Folic Acid Fortificant

Both white and yellow corns ( Zea mays var. indentata ) fermented by Bifidobacterium brevis has potential use as fortificant of natural folic acid. This experiment activity aims to find out difference in characteristic between concentrate powder of white corn and yellow corn fermented by Bifidobacterium brevis subsequently through processes of nixtamalization with lime solution Ca(OH) 2 , fermentation by B. brevis culture, microfiltration (MF) (pore size 0.15 µm) by means of standard dead-end stirred filtration cell (DESFC) at room temperature, rotation speed 300 rpm and 300 rpm, and trans membrane pressure (TMP) 20 and 40 psia for 30 minutes, respectively, and drying at 50 °C for 36 hours. The result of experiment showed that a series of processes mentioned above gave difference in characteristic from two types of biomass concentrate powder with compositions of folic acid 565.35 µg/mL and 614.92 µg/mL, total solids 96.60 mg/mL and 96.76 mg/mL, dissolved protein 0.19 mg/mL and 0.30 mg/mL, reducing sugars 1.718 mg/mL and 59.300 mg/mL, total sugars 2.54 mg/mL and 59.00 mg/mL, and total acid 0.29 % and 0.41%, respectively. In this condition, biomass concentrate powder of A and B displayed domination of folic acid monomer with molecular weight (MW) 442.07 Dalton (Da.) and 442,72 Da. at relative intensity 75 % and 100 %, particles size 1115.1 nm and 461.0 nm, and Index Particles 0.827 and 0.653, respectively.