Open AccessSynthesis, characterization and cytotoxicity of chitosan-coated Fe3O4 nanoparticles functionalized with ascorbic acid for biomedical applications
Author(s) -
Isabella M. Lourenço,
Milena T. Pelegrino,
Joana C. Pieretti,
Gislaine Patricia Andrade,
Giselle Cerchiaro,
Amedea B. Seabra
Publication year - 2019
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1323/1/012015
Subject(s) - ascorbic acid , nanocarriers , biocompatibility , superparamagnetism , nuclear chemistry , nanoparticle , chitosan , cytotoxicity , chemistry , nanomaterials , nanotechnology , materials science , organic chemistry , in vitro , biochemistry , physics , food science , magnetization , quantum mechanics , magnetic field
Iron oxide magnetic nanoparticles (Fe 3 O 4 NPs) are used to drive and to promote sustained release of drugs in target sites. Biocompatibility and superparamagnetic behaviour are important features to the successful biomedical applications of Fe 3 O 4 NPs. In this study, Fe 3 O 4 NPs were synthesized by the co-precipitation method and coated with chitosan (CS) containing ascorbic acid (AA), allowing formation of Fe 3 O 4 @CS-AA NPs. The antioxidant AA was used as a drug model. The synthesized NPs were characterized by different techniques. The results showed the formation of spherical nanoparticles with average diameter of 67.22 ± 0.82 nm, at solid state, as analysed by atomic force microscopy (AFM). The NPs were found to have a superparamagnetic behaviour at room temperature, and the presence of CS-AA on the surface of Fe 3 O 4 NPs did not affect the superparamagnetic behaviour of the nanoparticles. The in vitro AA release assay showed a sustained release of the model drug from Fe 3 O 4 @CS-AA NPs for at least 48 h. In addition, cytotoxicity assays for Fe 3 O 4 NPs and Fe 3 O 4 @CS-AA NPs did not show significant toxicity towards mammary epithelium (MCF-10A) cell line after 24 h of incubation. This present study demonstrated the successful synthesis of superparamagnetic and biocompatible Fe 3 O 4 @CS-AA NPs, which are able to release the model drug in a sustained manner. Thus, this nanomaterial might act as a nanocarrier in target drug release.