Open AccessAntibacterial activity of Saccharum officinarum leaves extract against food-borne disease
Author(s) -
Alfi Amalia,
Krispinus Kedati Pukan,
Nur Aini Setyawati,
Talitha Widiatningrum,
Umi Khasanah
Publication year - 2019
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1321/3/032043
Subject(s) - saccharum officinarum , escherichia coli , staphylococcus aureus , petroleum ether , bacteria , antibacterial activity , food science , ampicillin , microbiology and biotechnology , biology , chemistry , botany , biochemistry , genetics , chromatography , extraction (chemistry) , gene
Food-borne diseases are mainly caused by germs contaminated food which deliver serious illnesses such as intoxication, infection or combination of both. The germs could be Escherichia coli and Staphylococcus aureus. Previous study revealed Saccharum officinarum baggase competence in depressing these bacteria growth. Leaf should afford a better role for having no carbohydrate deposition. For that reason, its petroleum ether extract was prepared in a dilution series of 100 %, 75 %, 50 % or 25 % and used for antibacteria evaluation by paper disc method. The result indicated diminished growth of both bacteria by 100 % and 75 % extract, though not as effective as the positive control containing ampicillin. Furthermore, the Gram-positive bacteria was deprived more than the negative one. GCMS chromatogram depicted some fatty acid appearances that might contribute to the antimicrobe activity. A future study of antibacterial effect of each isolated compound should be managed. However, this study has already verified that the petroleum ether extract of Saccharum officinarum leaves were found to be an antibacterial agent against Escherichia coli and Staphylococcus aureus.