Open AccessTopical anti-inflammatory activity of n-hexane extract of santalum album linn leaves on rat ear oedema induced by croton oil
Author(s) -
Ni Made Puspawati,
Wiwik Susanah Rita
Publication year - 2019
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1321/2/022033
Subject(s) - croton oil , croton , santalum album , hexane , anti inflammatory , traditional medicine , inflammation , chemistry , medicine , pharmacology , biology , botany , chromatography
Santalum album Linn ( S.album ) has been traditionally used to treat inflammation. This research aimed to evaluate topical anti-inflammatory activity of nhexane extract of S.album leaves on croton-oil induced rat ear oedema. The anti-inflammatory activity test was done using 25 Wistar rats which divided randomly into five groups. Group 1 (negative control, croton oil 35% in acetone only), group 2 (positive control, hydrocortisone drug 2.5% and croton oil), and groups 3, 4, and 5 treated with 10, 20, and 40 mg/ear of n -hexane extracts respectively along with the croton oil. The rat ear-oedema thickness was measured manually using micrometre after 6 hours of observation. The topical application of n -hexane extract of S.album leaf at doses 10 and 20 mg/ear, and hydrocortisone significantly reduced croton-oil induced rat ear oedemawith percentage inhibition of 36, 52, and 73 % respectively. However, at a higher dose of 40 mg/ear, it did not significantly inhibit inflammation as compare to negative control. Histology analysis result also revealed that n hexane extract at doses 10 and 20 mg/ear, and hydrocortisone decreased significantly infiltration of leukocytes. Based on this result, it can be concluded that n -hexane extract of Santalun album Linn leaf showed topical anti-inflammatory activity in a dose dependent manner.