Open AccessQualitative analysis, total phenolic content, FT-IR and GC-MS characterisation of Canna indica: bioreducing agent for nanoparticles synthesis
Author(s) -
Oladotun P. Bolade,
Anuoluwa Abimbola Akinsiku,
Alaba Oladipupo Adeyemi,
Gbenga E. Jolayemi,
Akan B. Williams,
Nsikak U. Benson
Publication year - 2019
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1299/1/012135
Subject(s) - chemistry , phenols , phytochemical , mass spectrometry , fourier transform infrared spectroscopy , ethanol , gas chromatography–mass spectrometry , chromatography , infrared spectroscopy , gas chromatography , terpenoid , nuclear chemistry , organic chemistry , stereochemistry , biochemistry , physics , quantum mechanics
Within the framework of determining capping and stabilizing bioactive components present in Canna indica towards nanoparticles synthesis, phytochemical screening, total phenolic content, infrared spectroscopy and chromatographic characterisation were carried out on the locally sourced plant. Extracts were prepared from C. indica leaves using ethanol, de-ionised water (DW) and ethanol/DW in ratio 1:1. Qualitative screening showed the presence of saponins, alkaloids, terpenoids, phenols and coumarins. Highest total phenolic content (TPC) was observed in the aqueous fraction and least in ethanol fraction. Characterisation was carried out using Fourier Transform - Infrared spectroscopy (FT-IR) and Gas Chromatography - Mass Spectrometry (GC-MS). Absorption bands observed from FT-IR analysis showed presence of aromatic O-H stretch (3300 cm −1 ) and aromatic C=C stretch (1451 and 1640 cm −1 ) respectively. GC-MS analysis of ethanolic extract indicated the presence of dl-.alpha.-tocopherol – a phenolic compound.