Open AccessMolecular Study and Phylogenetic Analysis of Middle East Respiratory Syndrome Corona Virus (MERSCoV) in Camel and Human
Author(s) -
Saba F Alsalihi,
Ali Jawad,
Mohsen Alrodhan
Publication year - 2019
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1294/6/062097
Subject(s) - middle east respiratory syndrome , phylogenetic tree , amplicon , peninsula , middle east , virology , virus , genetic analysis , biology , polymerase chain reaction , veterinary medicine , gene , geography , covid-19 , genetics , medicine , archaeology , ecology , disease , infectious disease (medical specialty)
Middle East Respiratory Syndrome Corona Virus (MERSCoV) have been reported in Arabian peninsula and sporadic cases in Europe and Asia. This study was conducted to evaluate the genetic analysis of this virus in human and camel at the first time in Iraq. Two hundred samples were collected from camels and human who suffering from respiratory symptoms, these samples treated with RNA extraction kit then amplification the genetic material by PCR which give 5% positive results. The amplicon then sequenced, registration in gene bank of NCBI for getting accession numbers. The local strains give close relationship with neighbor countries as Saudi Arabia and Jordan strains when using MEGA analysis software.