Open AccessThe effects of Fusarium graminarum silver nanoparticles on leishmania tropica
Author(s) -
Omar Taher Mohammed,
Roua Jamal Abdulkhaliq,
Sabaa Taher Mohammed
Publication year - 2019
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1294/6/062075
Subject(s) - amastigote , leishmania tropica , in vitro , leishmania , viability assay , biology , chemistry , parasite hosting , microbiology and biotechnology , horticulture , biochemistry , computer science , world wide web
The present study aims to evaluate the in vitro antileishmanial activities of biosynthesis silver nanoparticles from Fusarium graminarum and to compare its efficacy with a pentostam drug against Leishmania Tropica .This nanaoparticles (20-40-80-100 μg/mL) was evaluated in vitro against promastigote and intracellular amastigote forms of L. tropica also measured the viability by use MTTassy. Its significantly ( < 0.05) inhibited the growth rate of promastigote the results showed decrease in number of parasite after 24hr was(1816.6,1300,541.6, ×10 4 ) cell\ml respectively, compared with pentostam and control was(1558.3 and 2441.6,308,3×10 4 ) cell\ml respectively. whereas after 48hr the number of parasite became(1583.3,1166.6,450,225×10 4 ) cell\ml respectively compared with pentostam and control was(1066.6 and 3166.6×10 4 ) cell\ml respectively, also observed (100 μg/mL)more effects on multiplication ofamastigote after 24hr become only 1.6parasite compared with pentostam and control was(3.3,7 parasites).The results of viability also revealed that the nanoparticles Cytotoxicty effect dependent on concentration and reduce to reach (23% ). Obtained findings also provide the scientific evidences that fungi could be used in the biosynthesis nanoparticles and treatment of CL.