Open AccessGrowth analysis of Microsporum canis using husk rice (Oryza Sativa L. CV. Ciherang) as a replacement for media Sabouroud Dextrose Agar (SDA)
Author(s) -
Haidir Syafrullah,
Anita Oktari,
Y. Supriatin,
Muhammad Husni
Publication year - 2019
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1280/2/022004
Subject(s) - microsporum canis , husk , potato dextrose agar , agar , conidium , biology , oryza sativa , hypha , inoculation , food science , horticulture , veterinary medicine , botany , microbiology and biotechnology , bacteria , antifungal , medicine , biochemistry , genetics , gene
Rice husk is a hard layer covering caryopsis which consists of two parts called the lemma and palea which are interconnected in the milling process. Rice husk is categorized as biomass that can be used for variety of needs such as raw materials and animal feed. Rice husk containing sources of carbohydrates and proteins which are the factors those support the growth of fungi. Sabouroud Dextrose Agar (SDA) is medium that could be used for the growth of fungi. Experimental research had been carried out by inoculating Microsporum canis in alternative media such as rice husk. This study aimed to compare the growth of Microsporum canis in alternative media and control media. It has been done by varying concentrations of rice husk media into 4.5%, 5%, and 5.5%. Observations done in 14 days, at room temperature with macroscopic examination each day by measuring the colony diameter using calipers. Microscopic examinations measure the length of macroconidia and Microsporum canis hyphae using microscope equipped with microoculary lens by staining using Lactophenol Cotton Blue (LPCB). Observations processed statistically using one-way ANOVA. From the result of the statistical test was concluded that rice husk powder concentration of 5.5% is the optimum concentration for the growth of Microsporum canis and can be used as a replacement media for Sabouroud Dextrose Agar (SDA).