Open AccessSIMULATION ASSESSMENT OF THE PROTECTIVE ROLE OF VITAMIN C AND E AGAINST CYTOTOXIC EFFECTS OF CYCLOPHOSPHAMIDE IN RATS TONGUE MUCOSA: HISTOLOGICAL AND IMMUNOHISTOCHEMICAL STUDY
Author(s) -
Ashty Omer Saleh,
Ameera Kamal Khleel,
Jasim Almahana
Publication year - 2019
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1279/1/012014
Subject(s) - cyclophosphamide , mucositis , immune system , proliferating cell nuclear antigen , vitamin e , immunohistochemistry , medicine , vitamin c , tongue , saline , vitamin , apoptosis , gastroenterology , pathology , immunology , toxicity , biology , chemotherapy , antioxidant , biochemistry
Objectives: Oral mucositis was found to be one of the most common side effects of cyclophosphamide use. The present study was designed to evaluate the effectiveness of vitamin C or E as a treatment for the induced cyclophosphamide tongue mucositis.Materials and Methods: Eighty rats were randomly divided into two equal groups: The control group was intraperitoneally injected by physiological saline and were grouped randomly into four equal subgroups: Distilled water, corn oil, vitamin C (12 mg/kg/day), and vitamin E (40mg/kg/day) treatment groups. For induction of mucositis to the study group, a single dose of cyclophosphamide (300 mg/kg) was administered intraperitoneally to each animal, and the animals were also grouped randomly in to four subgroups in the same manner as in the control group. Five of the animals in each group were sacrificed at day four and the other five at day eight and the tongue was dissected for histological and immunohistochemical analysis.Results: In comparison with the cyclophosphamide/water treated group, vitamin C caused a non-significant increase in epithelial thickness, non-significant decrease in damage score and caspase-3 immune expression at day four (p>0.05), but a significant increase in Proliferating Cell Nuclear Antigen (PCNA) immune expression at day four and eight was seen (p<0.05). While vitamin E cause a significant increase in epithelial thickness, a significant decrease in damage score and caspase-3 immune expression at day four, and a significant increase in PCNA immune expression at day four and eight (p<0.05). Conclusion: Vitamin E is better than vitamin C in decreasing the severity of tongue mucositis induced by cyclophosphamide in rats.