Open AccessEvaluation ofPseudomonas sp.growth in culture medium using electrical impedance spectroscopy with two bipolar geometries
Author(s) -
J. E. Aranzazu-Osorio,
L. H. Alzate
Publication year - 2019
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1272/1/012014
Subject(s) - dielectric spectroscopy , capacitance , electrical impedance , analytical chemistry (journal) , bacterial growth , agar , materials science , electrode , chemistry , bacteria , chromatography , electrochemistry , electrical engineering , biology , genetics , engineering
The electrical properties of microbial culture have been studied by electrical impedance spectroscopy. Electrochemical changes in microbial systems are facilitated by the metabolic and proteolytic action of microorganisms. In this study, the growth of isolated bacteria compatible with genus Pseudomonas was evaluated by direct technique using electrical impedance spectroscopy with two electrode geometries. In these experiments a minimal salts medium with glucose as the energy source was used. The data of electrical parameters were correlated by counting viable cells using cetrimide agar. Cell growth in the culture medium was increased in capacitance, clearly describing the exponential phase of bacterial growth. The correlation of the viable cell count compared with the capacitance showed a high Pearson coefficient (>0.9). The bacterial growth can be estimated in culture medium using electrical parameters, such as capacitance using parallel plates electrode and parallel circuit equations.