Open AccessThe relationship between TNF-α gene polymorphism, pro-inflammatory cytokines and bone turnover markers in COPD patients with osteoporosis
Author(s) -
Radiyati Umi Partan,
Rachmat Hidayat
Publication year - 2019
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1246/1/012035
Subject(s) - osteoprotegerin , osteoporosis , copd , rankl , medicine , bone remodeling , tumor necrosis factor alpha , endocrinology , femoral neck , bone mineral , proinflammatory cytokine , gene polymorphism , genotype , immunology , receptor , biology , inflammation , activator (genetics) , gene , genetics
Tumour necrosis factor alpha (TNF-α) is an important regulator of bone metabolism. Polymorphisms in the promoter region of the TNF-α gene at position 308 have been identified. We investigated whether these polymorphisms and circulating TNF-a levels were related to BMD in osteoporosis caused by COPD. We conducted this study to analyse the relationship between genetic polymorphism of tumour necrosis factor (TNF)-a -308 G/A and levels of pro-inflammatory cytokines, bone turnover marker levels, and the incidence of osteoporosis in COPD patients. This study was conducted on 70 COPD patients. BMD and bone area of the femoral neck and lumbar spines were measured using dual energy X-ray absorptiometry (Stratos ®). Blood cytokines (TNF-a, interleukin (IL)-6, IL-17, IL-1b) and Ctelopeptide (CTX), receptor activator of nuclear factor kB (RANKL), and osteoprotegerin (OPG) were analysed using ELISA. Polymorphism of the TNF-α gene -308 G/A was assayed by PCR-RFLP. The levels of cytokines were significantly increased in the osteoporosis group compared to those without. Polymorphism was significantly different between COPD with osteoporosis and COPD without. The frequency of the GA and AA genotypes was significantly increased in patients with osteoporosis. To conclude, there is a relationship between the TNF-a -308 G/A polymorphism and high levels of TNF-a, IL-1β, IL-6, IL-17, CTX, and the incidence of osteoporosis in patients with COPD.